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Mice with allodynia were randomly administered with WAY-100635 (n = 6), MDL-72222 (n = 6), or an equivalent volume of the vehicles (saline, the vehicle of WAY-100635, n = 5 or 20% DMSO, the vehicle of MDL-72222, n = 5) 15 min prior to rosavin treatment at days 3 to 5. Cold sensitivity was assessed before antagonist administration (Before) and 30 min after rosavin application (After). Data are expressed as mean ± SEM; **p < 0.01, ***p < 0.001, vs. Before; by Bonferroni post-hoc test after two-way analysis of variance (ANOVA).

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: 5-HT 1A receptors mediate the analgesic effect of rosavin in a mouse model of oxaliplatin-induced peripheral neuropathic pain

doi: 10.4196/kjpp.2021.25.5.489

Figure Lengend Snippet: Mice with allodynia were randomly administered with WAY-100635 (n = 6), MDL-72222 (n = 6), or an equivalent volume of the vehicles (saline, the vehicle of WAY-100635, n = 5 or 20% DMSO, the vehicle of MDL-72222, n = 5) 15 min prior to rosavin treatment at days 3 to 5. Cold sensitivity was assessed before antagonist administration (Before) and 30 min after rosavin application (After). Data are expressed as mean ± SEM; **p < 0.01, ***p < 0.001, vs. Before; by Bonferroni post-hoc test after two-way analysis of variance (ANOVA).

Article Snippet: Pretreatment with WAY-100635 (5-HT 1A receptor antagonist, 0.16 mg/kg, i.p.; Sigma) or bemesetron (MDL-72222, a 5-HT 3 receptor antagonist, 1 mg/kg, i.p.; Tocris, Cookson, UK) was performed to determine potential 5-HT receptor subtypes that mediate the analgesic action of rosavin [ , ].

Techniques: Saline